Bench scale production of nicotinic acid using a versatile amide-hydrolysing Geobacillus subterraneus RL-2a isolated from thermal spring of Manikaran, India
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- 作者：Praveen Kumar Mehta ; Shashi Kant Bhatia ; Ravi Kant Bhatia ; Tek Chand Bhalla ; bhallatc@rediffmail.com" class="auth_mail
- 关键词：Acyl transfer activity ; Amidase ; Geobacillus subterraneus RL-2a ; Isolation ; Nicotinic acid ; Thermostable
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：July 2014
- 年：2014
- 卷：105
- 期：Complete
- 页码：58-65
- 全文大小：849 K
文摘
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Biotransformation of nicotinamide to nicotinic acid, in 1 l scale fed batch reactor.
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This is the first reported Geobacillus subterraneus species having amidase activity.
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This is first reported broad substrate spectrum amidase from Geobacillus sp.
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Amidase with deactivation constant (kd) and enzyme t1/2 of 4.15 × 10−5 s−1 and 6 h 4 min.
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The amidase showed activity at wide range of temperature (40–90 °C) and pH (4.5–13).