In vivo transgene expression using an adenoviral tetracycline-regulated system with neuron-specific enolase promoter
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- 作者：Bhattacharjee ; Abesh Kumar ; Ueyama ; Takehiko ; Kondoh ; Takeshi ; Hayashi ; Shigeto ; Abouelfetouh ; Ayman ; et. al.
- 关键词：Adenovirus ; Gene expression ; Green fluorescent protein ; Neuron ; Tetracycline-inhibitable transcription factor ; Tetracycline-responsive promoter
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2004
- 出版时间：May 14, 2004
- 年：2004
- 卷：317
- 期：4
- 页码：1144-1148
- 全文大小：240 K
文摘
A recombinant adenoviral tetracycline-regulated system with neuron-specific enolase (NSE) promoter was injected stereotaxically into the striatum of rat brains. The efficiency of in vivo transfection was quantified by counting the number of green fluorescent protein (GFP)-positive cells at 3 days, 1 week, and 4 weeks after injection. NeuN immunohistochemistry demonstrated that expression of γPKC-GFP was dominant (20–99 % ) in neuron and expression of γPKC-GFP in neuron was significantly higher in pups than adult rats. These results indicate that tetracycline-inhibitable transcription factor (tTA) can drive tetracycline-responsive promoter (TetOp) under the control of NSE promoter, thereby efficiently and selectively expressing γPKC-GFP in neurons in vivo.