Detection of prostate specific antigen with 3,4-diaminobenzoic acid (DBA)–H₂O₂–HRP voltammetric enzyme-linked immunoassay system

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• 作者：Shusheng Zhang ; Ping Du ; Feng Li
• 关键词：Electrochemical immunoassay ; Prostate specific antigen ; Horseradish peroxidase ; 3 ; 4-Diaminobenzoic acid
• 刊名：Talanta
• 出版年：2007
• 出版时间：15 June 2007
• 年：2007
• 卷：72
• 期：4
• 页码：1487-1493
• 全文大小：379 K

文摘

A new voltammetric enzyme-linked immunoassay system of 3,4-diaminobenzoic acid (DBA)–H₂O₂–horseradish peroxidase (HRP) for sensitive detection of prostate specific antigen (PSA) in human serum was present. In the proposed procedure, labelled HRP efficiently catalyzed the oxidation reaction of DBA by H₂O₂ and generated the electroactive product, 2,2′-biamino-4,4′-bicarboxyl azobiphenyl, which produced a sensitive second-order derivative linear sweep voltammetric peak at potential of −0.62 V (versus SCE) in Britton–Robinson (BR) buffer solution. The linear range for detection free HRP was from 4.0 × 10⁻¹² to 4.0 × 10⁻⁹ g ml⁻¹ and the detection limit was about 6.0 × 10⁻¹³ g ml⁻¹. The proposed new system could be used for detection of PSA ranging from 0.20 to 16.0 ng ml⁻¹ with a detection limit of 0.10 ng ml⁻¹, which was five times lower than that of the traditional o-phenylendiamine spectrophotometric enzyme-linked immunosorbent assay (ELISA) method. The proposed electrochemical ELISA method is simple, inexpensive, reproducible and sensitive, which shows potential for detecting PSA in clinical diagnosis.