Kaposi’s sarcoma herpesvirus load in biopsies of cutaneous and oral Kaposi’s sarcoma lesions

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• 作者：Fatemeh Pak ; Amos R. Mwakigonja ; Parviz Kokhaei ; Nahid Hosseinzadeh ; Pawan Pyakurel ; Ephata Kaaya ; Gordana Bogdanovic ; Galina Selivanova ; Peter Biberfeld
• 关键词：Kaposi’ ; s sarcoma ; KSHV/HHV-8 ; Real time PCR ; Immunohistochemistry
• 刊名：European Journal of Cancer
• 出版年：2007
• 出版时间：August 2007
• 年：2007
• 卷：43
• 期：12
• 页码：1877-1882
• 全文大小：372 K

文摘

To evaluate human herpesvirus 8/Kaposi’s sarcoma associated herpesvirus (HHV-8/KSHV) viral load in diagnostic, (formalin fixed, paraffinised) biopsies and patient serum during tumour progression of oral and cutaneous AIDS-related Kaposi’s sarcoma (AKS), and endemic Kaposi’s sarcoma (EKS) by a sensitive and specific quantitative real time polymerase chain reaction (qRT-PCR) assay.

Study design

Eighty six biopsies of both AKS (oral and cutaneous AKS, 68) and EKS (cutaneous EKS, 18) were evaluated by qRT-PCR and immunohistochemistry (IHC). The viral load in human tumour tissue and serum of some individual patients were compared.

Results

Higher viral load as well as frequency of latency-associated nuclear antigen (LANA)+ tumour spindle cells (SC) and number of LANA granules per SC was found in oral AKS compared to cutaneous AKS. Although few cases were available, serum viral load appeared to decrease compared to tumour tissue during KS progression.

Conclusions

The higher viral load in oral rather than cutaneous AKS is consistent with the well recognised reservoir function of the oral mucosa. Decrease of serum HHV-8 load during KS progression may indicate decreased virus release and/or increased virus clearance.