Identification of a sulfonoquinovosyldiacylglyceride from Azadirachta indica and studies on its cytotoxic activity and DNA binding properties

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• 作者：Ratna Chatterjee ; Omkar Singh ; Lalawmpuii Pachuau ; Shiba Prasad Malik ; Mausumi Paul ; Kakali Bhadra ; Santanu Paul ; Gopinatha Suresh Kumar ; Nirup Bikash Mondal ; Sukdeb Banerjee
• 关键词：Azadirachta indica leaves ; Sulfonoquinovosyldiacylglyceride ; Sulfonoglycolipid ; 2D NMR ; Lymphoblastic leukemia ; Binding to CT DNA
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2010
• 出版时间：15 November 2010
• 年：2010
• 卷：20
• 期：22
• 页码：6699-6702
• 全文大小：428 K

文摘

Chromatographic separation of the methanolic extract of the leaves of Azadirachta indica led to the isolation of a sulfonoglycolipid characterized as a sulfonoquinovosyldiacylglyceride (SQDG), by extensive 2D NMR and mass spectral analysis. SQDG induces apoptosis in a dose dependent manner with IC₅₀ 8.3 μM against acute lymphoblastic leukemia (ALL) MOLT-4 cell lines. The compound showed significant DNA binding properties as evidenced by the enhancement of melting temperature and perturbation of the characteristic B-form in CD evidence of calf thymus DNA. The DNA binding was also characterized by isothermal calorimetry where a predominantly enthalpy driven binding to CT DNA was revealed.