Reduced and mutant lysozyme refolding with lipid vesicles. Model study of disulfide impact on equilibria and dynamics
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- 作者：Weiying Zhua ; Robert Silversb ; 1 ; Harald Schwalbeb ; Timothy A. Keiderlinga ; tak@uic.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Disulfide ; Reduction ; Lipid vesicles ; Protein folding ; Kinetics ; CD ; IR ; Fluorescence
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1864
- 期：9
- 页码：1083-1092
- 全文大小：1133 K
文摘
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Reduced and Cys → Ala mutated lysozymes refold to more than native helicity in lipids.
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Refolded helical segments mostly insert in lipid bilayer, sheet parts on surface.
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Native lysozyme has only small secondary structure change with lipid vesicles.
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Reduced lysozyme refolding kinetics in lipid vesicles are faster than renaturation.
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Kinetics indicate that lipid interaction mechanism differs from that with surfactants.