Determination of 3¦Â-hydroxy-¦¤⁵-bile acids and related compounds in biological fluids of patients with cholestasis by liquid chromatography-tandem mass spectrometry

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• 作者：Tsuyoshi Murai^a ; ^{murai@hoku-iryo-u.ac.jp} ; Kana Oda^a ; Terutake Toyo^a ; Hiroshi Nittono^b ; Hajime Takei^b ; Akina Muto^b ; Akihiko Kimura^c ; Takao Kurosawa^a
• 关键词：3¦Â-Hydroxy-¦¤5-bile acids ; 3¦Â-Hydroxy-¦¤5-C27-steriod dehydrogenase/isomerase deficiency ; High-performance liquid chromatography&ndash ; electrospray ionization-tandem mass spectrometry
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2013
• 出版时间：1 April, 2013
• 年：2013
• 卷：923-924
• 期：Complete
• 页码：120-127
• 全文大小：744 K

文摘

A method for the determination of conjugated and unconjugated 3¦Â-hydroxy-¦¤⁵-bile acids and related bile acids in human urine and serum has been developed using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Calibration curves for the bile acids were linear over the range of 10-2000 pmol/mL, and the detection limit was less than 4 pmol/mL for all bile acids using selected reaction monitoring analysis. The bile acids in urine and serum samples from two patients with severe cholestatic liver disease were measured by this analytical method. Glycine-conjugated 3¦Â-hydroxy-¦¤⁵-bile acid 3-sulfates were determined to be the major bile acids in the urine and serum from patients with a 3¦Â-hydroxy-¦¤⁵-C₂₇-steriod dehydrogenase/isomerase deficiency or dysfunction.