Characterization of mucilage polysaccharides, arabinogalactanproteins and cell-wall hemicellulosic polysaccharides isolated from flax seed meal: A wealth of structural moieties

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• 作者：Sayani Ray^a ; Florence Paynel^b ; Claudine Morvan^b ; Patrice Lerouge^c ; Azeddine Driouich^c ; Bimalendu Ray^a ; ^{bimalendu_ray@yahoo.co.uk} ; ^{bimalendu.ray@gmail.com}
• 关键词：AEC ; anion exchange chromatography ; AGP ; arabinogalactan protein ; AX ; arabinoxylan ; CT ; fraction extracted with chelating agent ; CW ; cell-wall ; FM ; defatted flax meal ; HG ; homogalacturonan ; HPAEC ; high performance anion exchange chromatography ; HWE ; fract
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：2 April, 2013
• 年：2013
• 卷：93
• 期：2
• 页码：651-660
• 全文大小：688 K

文摘

The present study aimed at analyzing the structural features of seed mucilage and cell-wall polysaccharides which accounted for 41 % of the mass of flax meal (FM). A combination of high molar-mass mucilage-like polysaccharides (rhamnogalacturonan and arabinoxylan) was released from FM in water, together with arabinogalactan proteins and glucans. About half of FM homogalacturonans was extracted using a calcium chelator and boiling water. Hemicellulosic xyloglucans and xylans were further extracted with 1 M KOH, in ¡«13 % FM-sugars yield. Structural characterization of the xyloglucan using specific enzyme hydrolysis, ion exchange chromatography (HPAEC) and matrix assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectroscopy showed the presence of XXXG type xyloglucan, but also that of XXGG-structure, possibly characteristic of flax seeds. Hydrolysis of xylans with endo-(1 ¡ú 4)-¦Â-d-xylanase, and analysis of the neutral and acidic oligosaccharides by MALDI-TOF-MS showed that xylan consisted of ¦Â-(1 ¡ú 4)-linked-d-xylopyranose backbone with some zones (DP 5-7) substituted with 4-O-MeGlcA\GlcA\Glc residues.