Multifaceted analyses of the interactions between human heart type fatty acid binding protein and its specific aptamers

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• 作者：Ankana Kakoti ; Pranab Goswami ; ^{pgoswami@iitg.ernet.in}
• 关键词：Aptamer ; Limited proteolysis ; MALDI ; Human heart type fatty acid binding protein
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：3289-3299
• 全文大小：2011 K
• 卷排序：1861

文摘

Developed two specific aptamers against human heart type fatty acid binding protein. Aptamer-protein interactions were analyzed by a combinatorial approach. Aptamer-protein interactions were accompanied by change in their conformations. Aptamers with low Kd exhibited high ionic interactions. Aptamers with high Kd exhibit more synergistic conformational change.