The adverse effects of phoxim exposure in the midgut of silkworm, Bombyx mori
详细信息 查看全文
- 作者：ZhiYa Gu ; YiJun Zhou ; Yi Xie ; FanChi Li ; Lie Ma ; ShanShan Sun ; Yu Wu ; BinBin Wang ; JuMei Wang ; Fashui Hong ; WeiDe Shen ; Bing Li
- 关键词：Phoxim ; O ; O-diethyl O-(alpha-cyanobenzylideneamino) phosphorothioate ; B. mori ; Bombyx mori ; AChE ; acetylcholinesterase ; DGE ; digital gene expression ; LC50 ; lethal concentration ; TPM ; number of transcripts per million clean tags ; PMSF ; phenylmethanesulfonyl fluoride ; CYP ; cytochrome P450 ; CarE ; carboxylesterase ; GST ; glutathione-S-transferase ; EST ; esterase ; CytC ; cytochrome C ; PBR ; peripheral-type benzodiazepine receptor ; VDAC ; voltage-dependent anion-selective channel ; IMD ; immune deficiency ; P
- 刊名：Chemosphere
- 出版年：February, 2014
- 年：2014
- 卷：96
- 期：Complete
- 页码：33-38
- 全文大小：983 K
文摘
The silkworm is an important economic insect. Poisoning of silkworms by organophosphate pesticides causes tremendous loss to the sericulture. In this study, Solexa sequencing technology was performed to profile the gene expression changes in the midgut of silkworms in response to 24 h of phoxim exposure and the impact on detoxification, apoptosis and immune defense were addressed. The results showed that 254 genes displayed at least 2.0-fold changes in expression levels, with 148 genes up-regulated and 106 genes down-regulated. Cytochrome P450 played an important role in detoxification. Histopathology examination and transmission electron microscope revealed swollen mitochondria and disappearance of the cristae of mitochondria, which are the important features in insect apoptotic cells. Cytochrome C release from mitochondria into the cytoplasm was confirmed. In addition, the Toll and immune deficiency (IMD) signal pathways were all inhibited using qRT-PCR. Our results could help better understand the impact of phoxim exposure on silkworm.