Generation of a reliable full-length cDNA of infectiousTembusu virus using a PCR-based protocol
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- 作者：Te Liang ; Xiaoxiao Liu ; Shulin Cui ; Shenghua Qu ; Dan Wang ; Ning Liu ; Fumin Wang ; Kang Ning ; Bing Zhang1 ; Zhangdb@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Dabing Zhang1 ; zdb@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Duck ; Tembusu virus ; Full-length cDNA ; Virus rescue
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 February 2016
- 年：2016
- 卷：213
- 期：Complete
- 页码：255-259
- 全文大小：820 K
文摘
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A full-length TMUV cDNA is generated using a PCR-based protocol.
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Infectious viral particles are rescued from BHK-21 cells transfected with in vitro transcribed RNA.
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Genomic characterization reveals that the rescued virus is genetically indistinguishable from the parental virus except genetic markers.
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The rescued virus possesses similar growth properties in BHK-21 cells and virulence in mice with the parental virus.
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Overall the technical platform will be useful for future investigations on the molecular mechanisms involved in replication and pathogenesis of TMUV.