Enzyme-assisted Extraction and Enrichment of Galanthamine from Lycoris aurea

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• 作者：Chun-lian Tian^a ; ^{tianchunlian1970@163.com" class="auth_mail" title="E-mail the corresponding author} ; Peng Wang^a ; Ji-xin Qin^b ; Xiao-pan Liu^a ; Ke Song^a ; Zhuo-bing Xiao^a
• 关键词：cation exchange resin ; enzyme-assisted extraction ; galanthamine ; Lycoris aurea ; separation
• 刊名：Chinese Herbal Medicines
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：8
• 期：2
• 页码：182-188
• 全文大小：795 K

文摘

To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L₉ (3⁴) orthogonal array design and separation effect of cation exchange resin on galanthamine.

Methods

Cellulase and pectinase solution was used as the extraction solvent. The effects of pH value of enzyme, amount of complex enzyme, dissociation time, and enzymatic hydrolysis temperature on the extraction results were investigated.

Results

a30">The optimal conditions were obtained as follows: ratio of solid to liquid (g: mL) 1:10, pH value 4.5, amount of complex enzymes 4%, enzymatic hydrolysis temperature 50 °C, and reaction time 2.0 h. Under these conditions, the extraction yield of galanthamine was 0.0294%. In addition, D-001 cation exchange resin was selected for separation of galanthamine. The separation conditions were that adsorption flow rate was 3 BV/h with reagent of pH 2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia. After separation, the content of galanthamine was increased to 12.31%.

Conclusion

The results provide a reference for industrial production of galanthamine.