Click aspartic acid as H ILIC SPE material for selective enrichment of N-linked glycopeptides
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- 作者：Xiuling Li ; Guobin Shen ; FeiFang Zhang ; Bingcheng Yang ; Xinmiao Liang
- 关键词：Glycopeptides ; Hydrophilic interaction chromatography ; Aspartic acid ; Click chemistry ; Solid phase extraction
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：15 December, 2013
- 年：2013
- 卷：941
- 期：Complete
- 页码：45-49
- 全文大小：1456 K
文摘
A novel hydrophilic interaction chromatography (HILIC) material was developed via clicking aspartic acid onto silica gel (termed as Click AA). The material demonstrated highly hydrophilic property and was used as solid phase extraction sorbent for selective enrichment of glycopeptides. By optimization of extraction conditions, Click AA exhibited high selectivity to glycopeptides with various peptide length and different types of glycans, which was much superior to similar commercial products. The application of Click AA to simulated proteomic samples further proved its specialty toward glycopeptides.