Bioanalytical and instrumental analysis of thyroid hormone disrupting compounds in water sources along the Yangtze River

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• 作者：Wei Shi ; Xiaoyi Wang ; Guanjiu Hu ; Yingqun Hao ; Xiaowei Zhang ; Hongling Liu ; Si Wei ; Xinru Wang ; Hongxia Yu
• 关键词：Reporter gene assay ; Thyroid hormone receptor ; Agonist equivalents ; Antagonist equivalents ; l-3 ; 5 ; 3′ ; -Triiodothyronine ; Dibutyl phthalate
• 刊名：Environmental Pollution
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：159
• 期：2
• 页码：441-448
• 全文大小：407 K

文摘

Thyroid hormone (TH) agonist and antagonist activities of water sources along the Yangtze River in China were surveyed by a green monkey kidney fibroblast (CV-1) cell-based TH reporter gene assay. Instrumental analysis was conducted to identify the responsible thyroid-active compounds. Instrumentally derived l-3,5,3′-triiodothyronine (T₃) equivalents (T₃-EQs) and thyroid receptor (TR) antagonist activity equivalents referring to dibutyl phthalate (DBP-EQs) were calculated from the concentrations of individual congeners. The reporter gene assay demonstrated that three out of eleven water sources contained TR agonist activity equivalents (TR-EQs), ranging from 286 to 293 ng T₃/L. Anti-thyroid hormone activities were found in all water sources with the TR antagonist activity equivalents referring to DBP (Ant-TR-EQs), ranging from 51.5 to 555.3 μg/L. Comparisons of the equivalents from instrumental and biological assays suggested that high concentrations of DBP and di-2-ethylhexyl phthalate (DEHP) were responsible for the observed TR antagonist activities at some locations along the Yangtze River.