We developed software that eased the workload of biomedical laboratory personnel such that they do not have to do manual image segmentation or learn to use software that requires programming skills. The software made a correct segmentation in most of the cases and outperformed the intensity oriented baseline method written in ImageJ in 95% of comparisons. The baseline method estimated cell- and background areas by averaging dark background and bright foreground areas.
Our software can be used in the calculation of cell areas and extents in the case where immunolabeled cells are imaged with a fluorescent microscope. In the future the functionality of the program could be extended with machine learning methods that use the user actions as teaching material in the cases where automatic segmentation fails.