Light sheet microscopes: Novel imaging toolbox for visualizing life’s processes
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- 作者：John M. Heddleston ; Teng-Leong Chew ; chewt@janelia.hhmi.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：Light sheet microscopy ; Select plane illumination microscopy ; Bessel beam ; Lattice light sheet microscopy ; Whole organism imaging
- 刊名：International Journal of Biochemistry and Cell Biology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：119-123
- 全文大小：715 K
文摘
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Light sheet microscopy was first developed in 1902.
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It has been used in biological imaging since in 2003.
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Light sheet microscopes exist in many flavors; each are tailored to address a particular biological challenge (e.g. sample size, spatial resolution, imaging depth).
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Light sheet microscopy provides improved signal-to-noise, lower photobleaching, and lower phototoxicity than conventional widefield, confocal or two-photon techniques.