A strategy for enrichment of the bioactive sphingoid base-1-phosphates produced by Hypericum perforatum L. in a balloon type airlift reactor

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• 作者：You-Xun Jin ; Xi-Hua Cui ; Kee-Yoeup Paek ; Yong-Hyeon Yim
• 关键词：Sphingoid base-1-phosphate ; IMAC ; Hypericum perforatum ; LC&ndash ; MS/MS ; Adventitious roots
• 刊名：Bioresource Technology
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：123
• 期：Complete
• 页码：284-289
• 全文大小：575 K

文摘

An efficient enrichment method using immobilized metal affinity chromatography (IMAC) was developed for selective extraction of bioactive sphingoid base-1-phosphates (SB1Ps) from adventitious roots of Hypericum perforatum cultured in bioreactor. The phosphate-selective IMAC enrichment coupled with LC-MS/MS enabled sensitive analysis of low-abundance SB1Ps present in the root biomass, which would not be feasible otherwise due to severe interferences from complex biological matrices. The time-dependent growth rate and production of SB1Ps from adventitious roots were investigated. The level of phytosphingosine-1-phosphate, which was found to be the major SB1Ps, reached a maximum amount of 635.6 pmol per gram of dry weight after 3 weeks of culture and decreased between 3 and 5 weeks of culture subsequently. On the other hand, sphingosine-1-phosphate and sphinganine-1-phosphate were present at levels of 18.91 and 73.15 pmol per gram of dry weight, respectively, after a week of culture and their level decreased thereafter.