Sensing of biomolecular interactions using fluorescence complementing systems in living cells
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- 作者：Xian-En Zhanga ; zhangxe@ibp.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Zongqiang Cuib ; Dianbing Wanga
- 关键词：Biomolecular interaction ; Molecular biosensors ; Fluorescence ; Living cells ; In vivo ; Intracellular ; BiFC ; TriFC ; Optical window ; Near infrared
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：76
- 期：Complete
- 页码：243-250
- 全文大小：1096 K
文摘
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Sensing molecular interactions in live cells has great significance to cell biology.
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BiFC molecular biosensor is a simple and reliable method for in vivo applications.
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This review provides a comprehensive survey of BiFC method and its applications.
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Near infrared and brighter BiFC systems are promising for deep tissue imaging.
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Simultaneous detection of molecular events can be made with multicolor BiFC systems.