文摘
Proteins can populate a variety of conformational and oligomeric states. CD, SEC, MALDI-ToF and crystallography display limited access to equilibrium conformational diversity. Ion mobility spectrometry (IMS) evidenced dissimilar conformational and oligomeric distribution of insulin. IMS showed sensitive to variables such as solution composition and sequence variants. IMS-MS may be considered a tool in profiling the oligomeric and conformational plasticity of biopharmaceuticals.