Towards an adrenaline biosensor based on substrate recycling amplification in combination with an enzyme logic gate

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• 作者：Denise Molinnus^a ; ^bAuthor Vitae ; Maren Sorich^aAuthor Vitae ; Alexander Bartz^aAuthor Vitae ; Petra Siegert^aAuthor Vitae ; Holger S. Willenberg^cAuthor Vitae ; Fred Lisdat^dAuthor Vitae ; Arshak Poghossian^a ; ^bAuthor Vitae ; Michael Keusgen^eAuthor Vitae ; Michael J. Schö ; ning^a ; ^b ; ^{schoening@fh-aachen.de" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Adrenaline ; Biosensor ; Laccase ; Glucose dehydrogenase ; Substrate recycling ; Enzyme logic gate
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：237
• 期：Complete
• 页码：190-195
• 全文大小：1514 K

文摘

An amperometric biosensor using a substrate recycling principle was realized for the detection of low adrenaline concentrations (1 nM) by measurements in phosphate buffer and Ringer’s solution at pH 6.5 and pH 7.4, respectively. In proof-of-concept experiments, a Boolean logic-gate principle has been applied to develop a digital adrenaline biosensor based on an enzyme AND logic gate. The obtained results demonstrate that the developed digital biosensor is capable for a rapid qualitative determination of the presence/absence of adrenaline in a YES/NO statement. Such digital biosensor could be used in clinical diagnostics for the control of a correct insertion of a catheter in the adrenal veins during adrenal venous-sampling procedure.