Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
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- 作者：Yin Hui Chow1 ; 2 ; Yee Jiun Yap3 ; Pau Loke Show4 ; Joon Ching Juan5 ; Mohd Shamsul Anuar1 ; Eng-Poh Ng6 ; Chien-Wei Ooi7 ; ooi.chien.wei@monash.edu" class="auth_mail" title="E-mail the corresponding author ; Tau Chuan Ling8
- 关键词：Aqueous two-phase system ; Statistical mechanics ; Bioseparation ; Protein recovery ; Purification ; Immunoglobulin G
- 刊名：Journal of Bioscience and Bioengineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：122
- 期：5
- 页码：613-619
- 全文大小：506 K
文摘
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Phase composition and NaCl concentration affect the IgG partitioning significantly.
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Accumulation of IgG at the interface intensified as phase composition increased.
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This phenomenon was correlated with a linear relationship between ln G and Δ[PEG].
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A linear relationship between ln K and NaCl concentration was proposed.
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All the studied ATPSs were fitted well with the applied equation.