Photoluminescence detection of 2,4,6-trinitrotoluene (TNT) binding on diatom frustule biosilica functionalized with an anti-TNT monoclonal antibody fragment
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- 作者：Le Zhena ; Nicole Fordb ; Debra K. Galea ; Guritno Roesijadib ; c ; Gregory L. Rorrera ; rorrergl@engr.orst.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Antibody ; Biosensor ; Biosilica ; Diatom ; Photoluminescence ; Trinitrotoluene
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：79
- 期：Complete
- 页码：742-748
- 全文大小：1158 K
文摘
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Frustules are nanostructured, high-porous biosilica shells of diatom algae.
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Diatom frustules emit blue light (photoluminescence) upon UV excitation.
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Diatom frustules were functionalized with an anti-TNT (trinitrotoluene) antibody fragment.
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Selective detection based on TNT binding with antibody-functionalized frustule.
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Label-free detection based on photoluminescence quenching of bound electrophilic TNT.