Identification of a diacylglycerol acyltransferase 2 gene involved in pheromone biosynthesis activating neuropeptide stimulated pheromone production in Bombyx mori

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• 作者：Mengfang Du ; Songdou Zhang ; Bin Zhu ; Xinming Yin ; Shiheng An ; ^{anshiheng@yahoo.com.cn}
• 关键词：Bombyx mori ; Pheromone gland ; Diacylglycerol acyltransferase (DGAT) ; Sex pheromone
• 刊名：Journal of Insect Physiology
• 出版年：2012
• 出版时间：May, 2012
• 年：2012
• 卷：58
• 期：5
• 页码：699-703
• 全文大小：411 K

文摘

Diacylglycerol acyltransferase (DGAT) catalyzes the final step in triacylglycerol biosynthesis. In the present study, a DGAT2 gene from Bombyx mori was characterized. Temporal expression profiles indicated that BmDGAT2 steadily increased from 96 h before eclosion (?6 h) to an expression peak in the pheromone glands (PGs) of new-emerged female (0 h), a key stage for sex pheromone production. Spatial expression analysis revealed that the BmDGAT2 transcript was most richly expressed in PGs. Decapitation and subsequent methoprene, a juvenile hormone (JH) analog, treatment experiments revealed that JH had no influence on the expression of BmDGAT2 transcript before emergence, but inhibited the expression of BmDGAT2 transcript when administered to newly emerged adults. Further RNAi analysis confirmed that the decrease in BmDGAT2 mRNA level caused a significant reduction in sex pheromone production. Thus, DGAT2 is a key enzyme regulating B. mori sex pheromone synthesis and release.