Efficient biosynthesis of levan from sucrose by a novel levansucrase from Brenneria goodwinii

详细信息    查看全文

• 作者：Qian Liu^a ; Shuhuai Yu^a ; Tao Zhang^a ; Bo Jiang^a ; Wanmeng Mu^a ; ^b ; ^{wmmu@jiangnan.edu.cn}
• 关键词：Biosynthesis ; Brenneria goodwinii ; Fructan ; Levan ; Levansucrase
• 刊名：Carbohydrate Polymers
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：157
• 期：Complete
• 页码：1732-1740
• 全文大小：1581 K
• 卷排序：157

文摘

A novel levansucrase was characterized from B. goodwinii. The polysaccharide biosynthesized from sucrose was determined to be levan. The enzyme displayed maximal transfructosylation activity at pH 6.0 and 35 °C. The enzyme produced 185 g/L levan from 50% (w/v) sucrose. The molecular weight of the produced levan reached 1.3 × 108 Da.