Quantification of the degree of biotinylation of proteins using proteinase K digestion and competition ELISA
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- 作者：Theo Rispens ; t.rispens@sanquin.nl" class="auth_mail" title="E-mail the corresponding author ; Pleuni Ooijevaar-de Heer
- 关键词：Biotin ; Degree of biotinylation ; Anti-biotin antibody ; Inhibition assay
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：430
- 期：Complete
- 页码：61-63
- 全文大小：285 K
文摘
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Robust quantification of the degree of biotinylation of proteins is achieved using a straightforward competition elisa.
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The use of anti-biotin instead of streptavidin results in a convenient range of sensitivity.
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A proteinase K digestion step assures unbiased measurement of biotin.