Development of a widely applicable immunoassay for insulin in marine teleosts that regulates cross-reactivity using biotinylation and inhibits interference by plasma components
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- 作者：Tadashi Andoh andoh@fra.affrc.go.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：A9 ; ninth residue of the A-chain ; IGF-I ; insulin-like growth factor ; IgG ; Immunoglobulin G ; HPLC ; high-performance liquid chromatography ; NHS ; N-hydroxysuccinimide ; SEM ; standard error of the mean ; TR-FIA ; time-resolved fluoroimmunoassay
- 刊名：General and Comparative Endocrinology
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：226
- 期：Complete
- 页码：72-81
- 全文大小：1101 K
文摘
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An immunoassay for measuring insulin in teleosts was developed.
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This assay can be applicable to at least 6 teleosts that are phylogenetically far.
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The applicability was achieved by regulating immunoreactivity in a labeled ligand.
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This is the first report of plasma interference in insulin immunoassay in fish.
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Novel simple methods for inhibition of this interference were also developed.