Structural characterization of cold extracted fraction of soluble sulfated polysaccharide from red seaweed Gracilaria birdiae
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- 作者：Jeanny S. Maciel ; Luciano S. Chaves ; Bartolomeu W.S. Souza ; Dá ; rlio I.A. Teixeira ; Ana L.P. Freitas ; Judith P.A. Feitosa ; Regina C.M. de Paula
- 关键词：Gracilaria birdiae ; Polysaccharide ; NMR spectroscopy ; Agarose ; Sulfate ester
- 刊名：Carbohydrate Polymers
- 出版年：2008
- 出版时间：7 March 2008
- 年：2008
- 卷：71
- 期：4
- 页码：559-565
- 全文大小：427 K
文摘
Water soluble polysaccharide from Gracilaria birdiae cultivated along the northeast coast of Brazil was characterized by infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy. The composition of the polysaccharide in wt % was determined as: β-mCaps"">d-galp (50.3 % ), 3,6-anhydro-α-mCaps"">l-galp (40.5 % ) and -α-mCaps"">l-galp-6 sulfate (9.2 % ). The ratio of mCaps"">l/mCaps"">d units (β-mCaps"">d-galp units and 3,6-anhydro-α-mCaps"">l-galp + α-mCaps"">l-galp-6 sulfate) is that of an ideal agarose. The sulfate content calculated by S % accounts for 6.4 % . 1D and 2D NMR techniques were employed in order to assign the spin system of polysaccharide without partial degradation. The structure is composed of → 4-3,6-anhydro-α-mCaps"">l-galp (1 → 3)β-mCaps"">d-galp 1 → segments, with the possibility of a α-mCaps"">l-galp unit substituted at the 6-position by sulfate ester.