β-Galactofuranose-containing structures present in the cell wall of the saprophytic fungus Cladosporium (Hormoconis) resinae

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• 作者：Renata Calixto ; Bianca Mattos ; Vera Bittencourt ; Lí ; via Lopes ; Lauro Souza ; Guilherme Sassaki ; Thales Cipriani ; Maria Silva ; Eliana Barreto-Bergter
• 关键词：Cladosporium resinae ; Hormoconis resinae ; Peptidogalactomannan ; NMR spectroscopy ; Carbohydrate epitopes
• 刊名：Research in Microbiology
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：161
• 期：8
• 页码：720-728
• 全文大小：540 K

文摘

A peptidogalactomannan was isolated from mycelia of Cladosporium (Hormoconis) resinae and characterized using methylation–fragmentation analysis, partial acid hydrolysis and ¹H and ¹³C-NMR spectroscopy. The galactomannan component was a branched structure and consisted of a main chain containing (1→6)-linked α-d-Manp residues substituted at O-2 by side chains containing (1→2)-linked α-d-Manp residues. β-d-Galf residues were present as side chains of 3–4 units that are (1→5)-interlinked. This structure is very similar to a pGM isolated from Aspergillus fumigatus and differs from that of Cladosporium werneckii (currently named Hortaea werneckii), with this pGM and other fungal galactomannans having single terminal (1→6)-linked β-Galf residues. The importance of the carbohydrate moiety of Cladosporium resinae pGM in immunoassays was also demonstrated. On FACS examination, a decrease (60 % ) in rabbit serum anti- C. resinae binding to C. resinae conidia occurred when this serum had been previously incubated with pGMs from C. resinae and A. fumigatus or with mannoprotein from Candida parapsilosis, suggesting the presence of cross-reactive determinants in these fungi.