Effective gene transfer to melanoma cells using bacterial ghosts

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• 作者：Pavol Kudela ; Susanne Paukner ; Ulrike Beate Mayr ; Dana Cholujova ; Gudrun Kohl ; Zuzana Schwarczova ; Jozef Bizik ; Jan Sedlak ; Werner Lubitz
• 关键词：Melanoma ; Bacterial ghost ; DNA carrier ; Gene expression
• 刊名：Cancer Letters
• 出版年：2008
• 出版时间：8 April 2008
• 年：2008
• 卷：262
• 期：1
• 页码：54-63
• 全文大小：214 K

文摘

Bacterial ghosts (BG) are cell envelopes preparations of Gram-negative bacteria devoid of cytoplasmic content produced by controlled expression of PhiX174 plasmid-encoded lysis gene E. Eight melanoma cell lines were investigated for their capacity to bind and phagocyte BG derived from Escherichia coli NM522 and Mannheimia haemolytica A23. High capability to bind BG was observed in almost all of the analyzed cell lines, furthermore cells were able to take up BG independently of the used bacterial species. Further, transfection efficiency of BG loaded with DNA in vitro was measured. The Bowes cells exhibited a high expression level of GFP and the incubation of cells with plasmid loaded BG led up to 82 % transfection efficiency.