Comparison of 2 molecular assays and a serologic test in diagnosing Mycoplasma pneumoniae infection in paediatrics patients

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• 作者：Blanco Silvia^a ;   ; ^b ;   ; ¹ ;   ; ^{sblanc1976@gmail.com} ; Fuenzalida Loreto^a ;   ; ¹ ; Bas Albert^a ; Prat Cristina^a ;   ; ^b ; Ramí ; rez Aida^a ; Matas Lurdes^a ;   ; ^d ; Rodrigo Carlos^c ; Ausina Vicente^a ;   ; ^b
• 关键词：M. pneumoniae ; Serology ; PCR
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：71
• 期：4
• 页码：463-466
• 全文大小：167 K

文摘

Two commercial polymerase chain reaction (PCR) assays (a real-time PCR [Cepheid] and an oligochromatographic test [Speed-oligo]) and 1 serology test (Serodia-Myco II) for detecting Mycoplasma pneumoniae in nasopharyngeal aspirates and serum samples were studied. Among the 145 samples, 32 serum pairs were serologically positive for M. pneumoniae. Of these, in 30 nasopharyngeal aspirates, M. pneumoniae was detected using the real-time PCR assay and 25 using Speed-oligo, corresponding to a sensitivity of 93.7 % and 78.1 % , respectively. Among the 94 samples with negative serology, we only obtained 1 positive result by real-time PCR assay. In the group of samples from healthy children, no positive results were obtained.