Combined hemolymph fractionation, hemocyte spreading assay and mass spectroscopy allowed identification of growth blocking peptide (GBP) in Locusta migratoria.
Locust GBP, 28-mer peptide, is located at C-terminus of a 64 amino acid long precursor polypeptide (secreted protein = 64–minus signal peptide….).
GBP elicits locust hemocyte spreading and postpones adult molting.
Most tissues except hemocytes, gut and Malpighian tubules display high GBP expression.
Schistocerca gregaria contains a corresponding encoded peptide with a single amino acid substitution.
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64-mer precursor having C-terminal 28-mer Locusta migratoria LmGBP.
LmGBP elicits locust hemocyte spreading in vitro and in vivo.
LmGBP extends 5th instar growth period.
Most tissues except hemocytes, gut, Malpighian tubules strongly express LmGBP.