Preparation and investigation of a cross-linked hyaluronan nanoparticles system

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• 作者：Mó ; nika Maroda ; Magdolna Bodn&#xe1 ; r ; Szilvia Berkó ; Jó ; zsef Bakó ; G&#xe1 ; bor Erő ; s ; Erzs&#xe9 ; bet Cs&#xe1 ; nyi ; Piroska Szabó ; -R&#xe9 ; v&#xe9 ; sz ; John F. Hartmann ; Lajos Kem&
• 关键词：Hyaluronic acid ; Nanoparticle ; Nanosphere ; Cross-linking
• 刊名：Carbohydrate Polymers
• 出版年：2011
• 出版时间：30 January 2011
• 年：2011
• 卷：83
• 期：3
• 页码：1322-1329
• 全文大小：1141 K

文摘

The present paper describes the preparation and characterization of hydrophilic hyaluronan nanoparticles formed by cross-linking of hyaluronic acid (HA) with 2,2′(ethylenedioxy)bis(ethylamine) in the presence of water-soluble carbodiimide (CDI) in aqueous media. The particle size of cross-linked nanosystems was measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM), transmittance by UV–VIS spectrophotometry, gel permeation chromatography (GPC) and rheology to characterize the physico-chemical properties.

The aqueous nanosystems prepared were stable, transparent or mildly opalescent with values of transmittance above 91 % . It was observed, that a fraction of particles with size less than 20 nm, has been released during the purification by diafiltration. It was established that the hydrodynamic size of cross-linked HA nanoparticles can be controlled by variation of the reaction conditions such as concentration of HA, salt concentration of media, ratio of cross-linker and the final pH of the reaction mixture.