Imaging of organelles by electron microscopy reveals protein–protein interactions in mitochondria and chloroplasts

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• 作者：Natalya V. Dudkina ; Roman Kouř ; il ; Jelle B. Bultema ; Egbert J. Boekema
• 关键词：Electron microscopy ; Tomography ; Single particle electron microscopy ; Mitochondrion ; Chloroplast ; Supercomplex
• 刊名：FEBS Letters
• 出版年：2010
• 出版时间：18 June 2010
• 年：2010
• 卷：584
• 期：12
• 页码：2510-2515
• 全文大小：631 K

文摘

Ongoing progress in electron microscopy (EM) offers now an opening to visualize cells at the nanoscale by cryo-electron tomography (ET). Large protein complexes can be resolved at near-atomic resolution by single particle averaging. Some examples from mitochondria and chloroplasts illustrate the possibilities with an emphasis on the membrane organization. Cryo-ET performed on non-chemically fixed, unstained, ice-embedded material can visualize specific large membrane protein complexes. In combination with averaging methods, 3D structures were calculated of mitochondrial ATP synthase at 6 nm resolution and of chloroplast photosystem II at 3.5 nm.