Proteomic analysis of Chilo iridescent virus
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- 作者：İ ; kbal Agah İ ; nce ; Sjef A. Boeren ; Monique M. van Oers ; Jacques J.M. Vervoort ; Just M. Vlak
- 关键词：Chilo iridescent virus ; Invertebrate iridovirus 6 ; Proteomics ; LC-MS/MS
- 刊名：Virology
- 出版年：2010
- 出版时间：15 September 2010
- 年：2010
- 卷：405
- 期：1
- 页码：253-258
- 全文大小：466 K
文摘
In this first proteomic analysis of an invertebrate iridovirus, 46 viral proteins were detected in the virions of Chilo iridescent virus (CIV) based on the detection of 2 or more distinct peptides; an additional 8 proteins were found based on a single peptide. Thirty-six of the 54 identified proteins have homologs in another invertebrate and/or in one or more vertebrate iridoviruses. The genes for 5 of the identified proteins, 22L (putative helicase), 118L, 142R (putative RNaseIII), 274L (major capsid protein) and 295L, are shared by all iridoviruses for which the complete nucleotide sequence is known and may therefore be considered as iridovirus core genes. Three identified proteins have homologs only in ascoviruses. The remaining 15 identified proteins are so far unique to CIV. In addition to broadening our insight in the structure and assembly of CIV virions, this knowledge is pivotal to unravel the initial steps in the infection process.