Influence of ambient air pollution on global DNA methylation in healthy adults: A seasonal follow-up

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• 作者：Sofie De Prins ; Gudrun Koppen ; Griet Jacobs ; Evi Dons ; Els Van de Mieroop ; Vera Nelen ; Frans Fierens ; Luc Int Panis ; Patrick De Boever ; Bianca Cox ; Tim S. Nawrot ; Greet Schoeters
• 关键词：WBC ; white blood cells ; LINE-1 ; long interspersed nucleotide elements ; dC ; 2&prime ; -deoxycytidine ; 5mdC ; 5-methyl-2&prime ; -deoxycytidine ; % 5mdC ; percentage 5mdC ; NO2 ; nitrogen dioxide ; PM10 ; particulate matter with aerodynamic diameter  ; &le ;   ; 10&#
• 刊名：Environment International
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：59
• 期：Complete
• 页码：418-424
• 全文大小：361 K

文摘

Background

DNA methylation changes are potential pathways of environmentally induced health effects. We investigated whether exposure to ambient concentrations of NO₂, PM₁₀, PM_2.5 and O₃ and traffic parameters were associated with global DNA methylation in blood of healthy adults.

Methods

48 non-smoking adults (25 males) with a median age of 39 years were sampled in winter and summer. Global DNA methylation in whole blood ( % 5-methyl-2¡ä-deoxycytidine, % 5mdC) was analyzed with HPLC. Exposure to air pollutants at the home address was assessed using interpolated NO₂, PM₁₀, PM_2.5 and O₃ concentrations for various exposure windows (60- to 1-day moving average exposures and yearly averages) and GIS-based traffic parameters. Associations between pollutants and % 5mdC were tested with multiple mixed effects regression models.

Results

Average % 5mdC (SD) was 4.30 (0.08) in winter and 4.29 (0.08) in summer. Men had higher % 5mdC compared to women both in winter (4.32 vs. 4.26) and summer (4.31 vs. 4.27). When winter and summer data were analyzed together, various NO₂, PM₁₀ and PM_2.5 moving average exposures were associated with changes in % 5mdC (95 % CI) ranging from ? 0.04 (? 0.09 to 0.00) to ? 0.14 (? 0.28 to 0.00) per IQR increase in pollutant. NO₂, PM₁₀, PM_2.5 and O₃ moving average exposures were associated with decreased % 5mdC (95 % CI) varying between ? 0.01 (? 0.03 to 0.00) and ? 0.17 (? 0.27 to ? 0.06) per IQR increase in pollutant in summer but not in winter.

Conclusion

Decreased global DNA methylation in whole blood was associated with exposure to NO₂, PM₁₀, PM_2.5 and O₃ at the home addresses of non- adults. Most effects were observed for the 5- to 30-day moving average exposures.