- 作者：Laure Chevalier ; Cé ; cile Bos ; Cé ; line Gryson ; Catherine Luengo ; Sté ; phane Walr ; Daniel Tomé ; Yves Boirie ; Claire Gaudichon
- 关键词：Protein kinetics ; High-protein diet ; Anabolism ; Tissue-specific regulations ; Liver metabolism ; Muscle protein fractions
- 刊名：Nutrition
- 出版年：2009
- 出版时间：September 2009
- 年：2009
- 卷：25
- 期：9
- 页码：932-939
- 全文大小：330 K
ObjectiveHigh-protein diets give rise to increased amplitude in the diurnal cycling of protein gains and losses at the whole-body level, but the tissue localization and mechanisms underlying these metabolic adaptations remain unclear. We investigated tissue-specific responses to increasing protein intakes in rats.Methods
Protein synthesis rates (flooding dose with 13C-valine) and accretion were assessed in individual tissues of fasted or fed rats (n = 32) after a 2-wk adaptation to a normal- or high-protein (HP) diet.
Results
In livers of HP rats, a strong inhibition of protein synthesis rates (−34 % ) occurred in the fasted and fed states, whereas a higher protein content (+10 % ) was observed. In the kidneys, a slight inhibition of synthesis rates after the HP diet was also observed but remained without effect on kidney protein pool size. Stomach and skin protein synthesis rates were significantly increased under HP conditions, whereas protein anabolism in skeletal muscle remained insensitive to the dietary protein level. This was also true for specific muscle protein fractions: myosin, mitochondrial, or sarcoplasmic protein synthesis rates were influenced by neither the dietary protein level nor the nutritional status.
Conclusion
Modulation of protein kinetics and accretion by the HP diet is tissue-specific and the liver plays a critical role in such adaptations in a unique situation associating an inhibition of protein synthesis and protein pool expansion. The mechanisms underlying these changes and their physiologic incidence remain to be elucidated.