- 作者：Bongani Donnycath Tshabalala ; Kazeem Adekunle Alayande ; Saheed Sabiu ; Anofi Omotayo Tom Ashafa ; ashafaaot@ufs.ac.za" class="auth_mail" title="E-mail the corresponding author
- 关键词：Antimicrobial activity ; Anthelmintic activity ; Intestinal parasitic infections ; Phytochemical screening ; Gazania krebsiana
- 刊名：European Journal of Integrative Medicine
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：8
- 期：4
- 页码：533-539
- 全文大小：363 K
G. krebsiana’s antimicrobial activity was evaluated against human pathogenic bacteria and fungi using agar well dilution method, while its anthelmintic activity was tested against gastrointestinal nematodes of sheep using egg hatchability and larvae mortality assays.
<h4 id="absSec_3">Resultsh4>The extracts elicited varied degrees of antimicrobial activity with the ethanol extract showing the lowest minimum inhibitory concentrations (MICs) of 0.16–0.63 mg/mL. The activity of the water extract appeared to be the least with MICs ranging from 10 to 20 mg/mL on most of the isolates. At 10 mg/mL, both the ethanol and acetone root extracts of G. krebsiana caused 100% egg hatch inhibition, and competed well with Tramisol®. Similarly, except for ethanol extract, other leaf extracts displayed commendable and concentration-dependent anthelmintic activity of nearly 100% (90.70–93.70%) inhibitions and a corresponding 100% parasite mortality after 72 h exposure period at 10 mg/mL dose.
<h4 id="absSec_4">Conclusionh4>The observed effects could be adduced to the presence of active principles as revealed by the phytochemical analysis of the extracts. There is a need for in vivo studies to complement the present findings, the data so far presented have validated and lent scientific support to the applications of G. krebsiana as a vermifuge and antimicrobial agent by the Basotho people of the eastern Free State of South Africa.