Spectral and 3D model studies of the interaction of orphan human cytochrome P450 2U1 with substrates and ligands

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• 作者：Laura Dhers^a ; Nicolas Pietrancosta^a ; Lionel Ducassou^a ; Booma Ramassamy^a ; Julien Dairou^a ; Maryse Jaouen^a ; Franç ; ois André ; ^b ; Daniel Mansuy^a ; Jean-Luc Boucher^a ; ^{jean-luc.boucher@parisdescartes.fr}
• 关键词：AA ; arachidonic acid ; AS ; N-arachidonoylserotonin ; BSA ; bovine serum albumin ; CYP ; cytochrome P450 ; Deb ; debrisoquine ; DLPC ; 1 ; 2-Didodecanoyl-sn-glycero-3-phosphocholine ; Im ; imidazole ; IPTG ; isopropyl β-D-1-thiogalactopyranoside ; LA ; lauric acid ; Terfimidazole ; 1-(4-imidazolylphenyl)-4-(4-hydroxydiphenylmethyl)piperidin-1-yl)butan-1-one)
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：3144-3153
• 全文大小：2176 K
• 卷排序：1861

文摘

Few data are available on orphan human cytochrome P450 2U1. N-Ter truncated, soluble, human CYP2U1 was expressed in E. coli. UV–vis and EPR spectroscopy binding experiments of substrates and imidazoles were performed. Docking experiments in our 3D model explained the spectroscopic data.