- 作者：Jing Yuan ; Boping Zhou ; Yasuhito Tanaka ; Fuat Kurbanov ; Etsuro Orito ; Zuojiong Gong ; Liumei Xu ; Jian Lu ; Xiaoling Jiang ; Weizhen Lai ; et al.
- 关键词：HBV ; Genotype ; Subgenotype ; BCP mutation ; CP mutation ; PC mutation
- 刊名：Journal of Clinical Virology
- 出版年：2007
- 出版时间：June 2007
- 年：2007
- 卷：39
- 期：2
- 页码：87-93
- 全文大小：251 K
The association of hepatitis B virus (HBV) genotypes with clinical course of infection is increasingly recognized.
Objectives
In order to investigate the genetic diversity of HBV and its clinical implications, 241 HBV-infected patients including 34 with hepatocellular carcinoma (HCC) were enrolled in this study.
Methods
HBV genotyping was performed with an ELISA assay. HBV subgenotypes were determined by PCR-RFLP. HBV core promoter/precore/core mutations were analyzed by direct sequencing.
Results
The overall prevalence of HBV/B and C was 65 % and 33 % , respectively. Among HBV/C, 42 % were Cs/C1 and 58 % were Ce/C2. The HBV/C1 was only found in the patients originating from Southern China (p = 0.0001). Among HCC patients, HBV/C2 was only found in the elder age group (≥51 years; p < 0.05) and HBV/Ba was associated with young HCC patients (<35 years). Mutations associated with HCC were V1753 and T1762/A1764 (p < 0.01). The prevalence of the V1753 was higher in HBV/C1 strains (p < 0.04), A1898 was only found among HBV/C1 (p = 0.056). T1762/A1764 was frequently demonstrated in both subgenotypes. The T1858 (90 % ) and A1896 (40 % ) mutations were most frequent in HBV/C2 (p < 0.008).
Conclusions
HBV/C1 and HBV/C2 have distinct geographic distributions in China. V1753 in addition to T1762/A1764 double mutation in the basal core promoter region seems to be associated with HCC development, especially in the patients with HBV/C1.