Tuber borchii mycelial protoplasts isolation, characterization and functional delivery of liposome content, a new step towards truffles biotechnology

详细信息    查看全文

• 作者：Anna Poma ; Sabrina Colafarina ; Tania Limongi and Giovanni Pacioni
• 关键词：Mycorrhizal fungi ; Tuber borchii ; Protoplasts ; Liposomes
• 刊名：FEMS Microbiology Letters
• 出版年：2005
• 出版时间：15 December 2005
• 年：2005
• 卷：253
• 期：2
• 页码：331-337

文摘

The filametous ascomycete Tuber borchii is a plant-symbiotic ectomycorrhizal microrganism with an high value due to the production of hypogeous fruitbodies (truffles). The present work was undertaken to develop a procedure for the release of T. borchii viable protoplasts from Tuber mycelium, isolate ATTC 96540; several factors which affect the isolation, morphology and viability were examined and developed in order to improve applications of T. borchii protoplasts in morphological, biochemical and genetic investigations (protoplast fusion or transformation). Functional delivery of liposome content into T. borchii protoplasts has also been examined with a cytotoxic ribosome inactivator as saporin. T. borchii protoplasts incubation/fusion with saporin containing liposomes were made to demonstrate the absence of cell wall of 16 days cultured protoplasts.