Glycan structure of Gc Protein-derived Macrophage Activating Factor as revealed by mass spectrometry
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- 作者：Chad R. Borgesa ; b ; chad.borges@asu.edu" class="auth_mail" title="E-mail the corresponding author ; Douglas S. Rehderb ; 1 ; doug.rehder@gilead.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Vitamin D binding protein ; GcMAF ; Linear trisaccharide ; Disaccharide ; ESI-MS ; Linkage analysis
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：606
- 期：Complete
- 页码：167-179
- 全文大小：1309 K
文摘
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The primary O-glycan of GcMAF is a Gal-GalNAc-Thr disaccharide.
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The DBP O-glycan on the 1F and 1S human alleles is NeuNAc-(2-3)-Gal-(1-3)-GalNAc-Thr.
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The DBP O-glycan on the 1F and 1S human allele products lacks mannose.
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α-N-Acetylgalactosamindase (“nagalase”) cannot cleave the intact DBP O-glycan.