Identification and Expression of a ¦Â-actin Gene from Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae)

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• 作者：Hong-bo JIANG^a ;   ; ^{jhb8342@163.com} ; Guang-mao SHEN^a ; Wei DOU^a ; Pei-an TANG^a ;   ; ^b ; Yong-hua LIU^a ; An-wei ZHOU^a ; Jin-jun WANG^a ;   ; ^{jjwang7008@yahoo.com}
• 关键词：actin ; sequence analysis ; expression ; real-time PCR
• 刊名：Agricultural Sciences in China
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：10
• 期：9
• 页码：1391-1401
• 全文大小：595 K

文摘

A ¦Â-actin gene, Lib¦Â-actinl, from the psocid, Liposcelis bostrychophila, was isolated, sequenced, and expressed in Escherichia coli. The cDNA sequence was 1 281 bp in length and contained an open reading frame of 1 131 bp encoding 376 amino acids with a predicted molecular weight of 41.82 kDa. According to a BlastN search, the coding region shared the highest identity (97 % ) with Pediculus humanus actin 5C, while the deduced amino acid sequence was completely identical to a mutant of Drosophila melanogaster actin 5C. Comparison of the nucleotide and deduced amino acid sequences confirmed the high similarity between Lib¦Â-actinl and homologs in other insect species. The 3?untranslated region (3?UTR) of the Lib¦Â-actinl mRNA had a high A+U content (approximately 75 % ) and contained three repeats of the AUUUUUA and AUUUA motifs, which may play a role in regulating mRNA decay. The expression of Lib¦Â-actinl was further analyzed in insecticide induced and control psocids. The results indicated that there was no significant difference in expression of Lib¦Â-actinl between the induced and control groups, suggesting that Lib¦Â-actinl may be an appropriate internal control for the gene expression profiling in this insect. Furthermore, Lib¦Â-actinl was also heterologously expressed in Escherichia coli, which provided a basis to investigate the physiological functions of actin genes in the psocid.