- 作者：Yuanfang Lu ; Changhua Zhuo ; Bota Cui ; Zhengxia Liu ; Ping Zhou ; Yuanming Lu ; Baolin Wang
- 关键词：Colorectal cancer ; Lymph node metastasis ; Prognosis ; Quantitative real-time PCR analysis ; DNA repair ; TYMS
- 刊名：Clinical Biochemistry
- 出版年：2013
- 出版时间：October, 2013
- 年：2013
- 卷：46
- 期：15
- 页码：1478-1483
- 全文大小：403 K
Objective
The current study is to evaluate the effect of thymidylate synthase (TYMS) on lymph node metastasis (LNM) in Chinese colorectal cancer (CRC) patients, and develop potential LNM-associated biomarkers for CRC.Design and methods
Differences in TYMS gene expression between primary CRC with LNM (LNM CRC) and without LNM (non-LNM CRC) were assessed using quantitative real-time PCR analysis in 100 Chinese colorectal cancer patients. The relationship between clinicopathological parameters and prognosis of candidate biomarkers was also examined in the experiment.
Results
TYMS was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by real-time quantitative polymerase chain reaction. Overexpression of TYMS was significantly associated with LNM (P < 0.001), advanced TNM stage (P < 0.001), increased 5-year recurrence rate (P < 0.001) and decreased 5-year overall survival rate (P < 0.001). Univariate and multivariate analyses indicated that TYMS expression was an independent prognostic factor for recurrence and survival of CRC patients (P < 0.05).
Conclusions
TYMS effect on lymph node metastasis in CRC might serve as a potential biomarker for LNM and a prognostic factor in CRC. Over-expression of TYMS is a predicting factor to the poor outcome in clinical colorectal cancer patients.