Protein kinase A activation by retinoic acid in the nuclei of HL60 cells
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- 作者：Asako Sakai ; Masahiko Imai ; Katsuhiko Takahashi ; Shinya Hasegawa ; Masahiro Yamasaki ; Toshihiro Ohba ; Noriko Takahashi ; t-noriko@hoshi.ac.jp
- 关键词：PKA ; protein kinase A ; RA ; retinoic acid ; HEPES ; 4-(2-hydroxyethyl)-1-piperazinethanesulfonic acid ; FBS ; fetal bovine serum ; PBS ; phosphate-buffered saline ; EDTA ; ethylenediaminetetraacetic acid ; BSA ; bovine serum albumin ; DTT ; dithiothreitol ; RAR ; retinoic acid receptor ; 2D-PAGE ; two-dimensional polyacrylamide gel electrophoresis ; SDS ; sodium dodecyl sulfate ; CBB ; coomassie brilliant blue R-250 ; APL ; acute promyelocytic leukemia
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：1861
- 期：2
- 页码：276-285
- 全文大小：1513 K
- 卷排序：1861
文摘
Retinoic acid (RA) activates protein kinase A (PKA) during cell differentiation. The RIIα regulatory subunit of PKA is modified by RA in early-differentiation stage. RA alters intracellular localization of RIIα and the activation of PKA in cells. RA stabilizes RIIα protein, and increases levels of nuclear phosphorylated proteins. RA-binding may promote PKA stabilization and activation, and cell differentiation.