Cytosolic phospholipase A₂-driven PGE₂ synthesis within unsaturated fatty acids-induced lipid bodies of epithelial cells

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• 作者：Luciana S. Moreira ; Bruno Piva ; Luciana B. Gentile ; Fabio P. Mesquita-Santos ; Heloisa D'Avila ; Clarissa M. Maya-Monteiro ; Patrí ; cia T. Bozza ; Christianne B ; eira-Melo ; Bruno L. Diaz
• 关键词：Lipid droplet ; Epithelial cell ; cPLA₂-α ; PGE₂ ; Arachidonic acid ; Oleic acid ; p38 MAP kinase
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
• 出版年：2009
• 出版时间：March 2009
• 年：2009
• 卷：1791
• 期：3
• 页码：156-165
• 全文大小：451 K

文摘

Cytoplasmic lipid bodies (also known as lipid droplets) are intracellular deposits of arachidonic acid (AA), which can be metabolized for eicosanoid generation. PGE₂ is a major AA metabolite produced by epithelial cells and can modulate restoration of epithelium homeostasis after injury. We studied lipid body biogenesis and their role in AA metabolic pathway in an epithelial cell line derived from normal rat intestinal epithelium, IEC-6 cells. Lipid bodies were virtually absent in confluent IEC-6 cells. Stimulation of confluent IEC-6 cells with unsaturated fatty acids, including AA or oleic acid (OA), induced rapid lipid body assembly that was independent on its metabolism to PGE₂, but dependent on G-coupled receptor-driven signaling through p38, PKC, and PI3K. Newly formed lipid bodies compartmentalized cytosolic phospholipase (cPL)A₂-α, while facilitated AA mobilization and synthesis of PGE₂ within epithelial cells. Thus, both lipid body-related events, including highly regulated biogenesis and functional assembly of cPLA₂-α-driven enhanced AA mobilization and PGE₂ production, may have key roles in epithelial cell-driven inflammatory functions, and may represent relevant therapeutic targets of epithelial pathologies.