May Sonic Hedgehog proteins be markers for malignancy in uterine smooth muscle tumors?

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• 作者：Natalia Garcia ; MSc^a ; Nilo Bozzini ; MD ; PhD^a ; Glauco Baiocchi ; PhD^b ; Isabela Werneck da Cunha ; MD ; PhD^b ; Gustavo Arantes Maciel ; MD ; PhD^a ; Jos&eacute ; Maria Soares Junior ; MD ; PhD^a ; Fernando Augusto Soares ; MD ; PhD^b ; Edmund Chada Baracat ; MD ; PhD^a ; Katia Candido Carvalho ; PhD^a ; ^{carvalhokc@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sonic Hedgehog ; Immunohistochemistry ; Tissue microarray ; USMTs ; Prognosis
• 刊名：Human Pathology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：50
• 期：Complete
• 页码：43-50
• 全文大小：1143 K

文摘

Several studies have demonstrated that the Sonic Hedgehog signaling pathway (SHH) plays an important role in tumorigenesis and cellular differentiation. We analyzed the protein expression of SHH pathway components and evaluated whether their profile could be useful for the diagnosis, prognosis, or prediction of the risk of malignancy for uterine smooth muscle tumors (USMTs). A total of 176 samples (20 myometrium, 119 variants of leiomyoma, and 37 leiomyosarcoma) were evaluated for the protein expression of the SHH signaling components, HHIP1 (SHH inhibitor), and BMP4 (SHH target) by immunohistochemistry. Western blot analysis was performed to verify the specificity of the antibodies. We grouped leiomyoma samples into conventional leiomyomas and unusual leiomyomas that comprise atypical, cellular, mitotically active leiomyomas and uterine smooth muscle tumors of uncertain malignant potential. Immunohistochemical analysis showed that SMO, SUFU, GLI1, GLI3, and BMP4 expression gradually increased depending on to the histologic tissue type. The protein expression of SMO, SUFU, and GLI1 was increased in unusual leiomyoma and leiomyosarcoma samples compared to normal myometrium. The inhibitor HHIP1 showed higher expression in myometrium, whereas only negative or basal expression of SMO, SUFU, GLI1, and GLI3 was detected in these samples. Strong expression of SHH was associated with poorer overall survival. Our data suggest that the expression of SHH proteins can be useful for evaluating the potential risk of malignancy for USMTs. Moreover, GLI1 and SMO may serve as future therapeutic targets for women with USMTs.