Profile of NF-¦ÊBp(65/NF¦ÊBp50) among prostate specific antigen sera levels in prostatic pathologies

详细信息    查看全文

• 作者：Y. Bouraoui ; A. Ben Jemaa ; G. Rodriguez ; N. Ben Rais ; B. Fraile ; R. Paniagua ; S. Sellemi ; M. Royuela ; R. Oueslati
• 关键词：NF-¦ÊB (p50/p65) ; PSA ; Prostatic pathologies ; Inflammation ; Cancer
• 刊名：Pathologie Biologie
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：60
• 期：5
• 页码：301-305
• 全文大小：690 K

文摘

Aim

The aim of this work was to characterise the immunoexpression of NF-¦ÊB (p50/p65) in human prostatic pathologies and to study its profiles of activation among sera prostate specific antigen antigen (PSA) according the three groups: 0-4 ng/mL, 4-20 ng/mL and > 20 ng/mL.

Patients and methods

Twenty-four men with benign prostate hyperplasia (BPH); 19 men with prostate cancer (PC) and five men with normal prostates (NP). Immunohistochemical and western blot analysis was performed. Serum levels of PSA were assayed by immulite autoanalyser.

Results

In BPH and PC samples, immunoexpressions were observed for NF-¦ÊBp65 and NF-¦ÊBp50; while in NP samples, only were detected NF-¦ÊBp50. PC samples showed immunoreactions to NF-¦ÊBp65 and NF-¦ÊBp50 more intense (respectively 24.18 ¡À 0.67 and 28.23 ¡À 2.01) than that observed in BPH samples (respectively18.46 ¡À 2.04 and 18.66 ¡À 1.59) with special localisation in the nucleus. Different profiles of NF-¦ÊBp65 immunoexpressions were observed and BPH patients with sera PSA levels between 0-4 ng/mL presented a significant weak percentage compared to BPH patients with sera PSA levels between 4-20 ng/mL and > 20 ng/mL. No immunoreactions to NF-¦ÊBp65 were observed in PC patients with sera PSA levels between 4-20 ng/mL.

Conclusion

The sensibility of both NF-¦ÊB and PSA to inflammation allowed confirming the relationship between these two molecules and its involvement in prostatic diseases progression (inflammatory and neoplasic).