Wear particle-mediated expressions of pro-inflammatory cytokines, NF-¦ÊB and RANK were impacted by lanthanum chloride in RAW264.7 cells

详细信息    查看全文

• 作者：Min DAI^a ; ^{daimin@medmail.com.cn} ; Chuan JIANG^a ; Xiang LIU^a ; Zhe LI^a ; Xigao CHENG^b ; Yang ZOU^a ; Tao NIE^a
• 关键词：lanthanum chloride ; wear particle ; osteoclastogenesis ; inflammation ; pro-inflammatory cytokine ; NF-¦ÊB ; RANK ; M-CSFR ; rare earths
• 刊名：Journal of Rare Earths
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：31
• 期：5
• 页码：531-540
• 全文大小：1089 K

文摘

To explore the impact of different concentrations of lanthanum chloride (LaCl₃) on critical components of wear particle-mediated signaling pathways in inflammation and osteoclastogenesis, RAW264.7 cells were naturally divided into eight groups and analyzed by CCK-8 assay, flow cytometry, ELISA, RT-PCR and western blot after treatments. The results showed that three concentrations of LaCl₃ had no influence on viability of RAW264.7 cells and down-regulated receptor activator of nuclear factor ¦ÊB (RANK) instead of macrophage colony-stimulating factor receptor (M-CSFR). Additionally, 2.5 and 10 ¦Ìmol/L LaCl₃ could significantly inhibit gene and protein levels of pro-inflammatory cytokines (tumor necrosis factor-¦Á and interleukin-1¦Â, i.e., TNF-¦Á and IL-1¦Â and NF-¦ÊB/p65, but 100 ¦Ìmol/L LaCl₃ did not exert an obvious inflammation-inhibiting effect, and even induced inflammation. In conclusion, these findings demonstrated that LaCl₃ was able to suppress wear particle-induced inflammation and activation of NF-¦ÊB in a certain range of concentrations in vitro and mainly decrease the expression of RANK, but not M-CSFR, all of which were generally recognized to play a pivotal role in osteoclastogenesis.