Characterization of substrate and product specificity of the purified recombinant glycogen branching enzyme of Rhodothermus obamensis

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• 作者：Xavier Roussel^a ; Christine Lancelon-Pin^b ; Anders Viksø ; -Nielsen^c ; Agnè ; s Rolland-Sabaté ; ^d ; Florent Grimaud^e ; ^f ; ^g ; Gabrielle Potocki-Vé ; ronè ; se^e ; ^f ; ^g ; Alain Bulé ; on^d ; Jean-Luc Putaux^b ; Christophe D'Hulst^a ; ^{christophe.dhulst@univ-lille1.fr}
• 关键词：Branching enzyme ; Rhodothermus obamensis ; Glycogen ; Dextrin ; Polyglucan ; In vitro synthesis
• 刊名：Biochimica et Biophysica Acta (BBA)/General Subjects
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：1830
• 期：1
• 页码：2167-2177
• 全文大小：1941 K

文摘

Background

Glycogen and starch branching enzymes catalyze the formation of ¦Á(1 ¡ú 6) linkages in storage polysaccharides by rearrangement of preexisting ¦Á-glucans. This reaction occurs through the cleavage of ¦Á(1 ¡ú 4) linkage and transfer in ¦Á(1 ¡ú 6) of the fragment in non-reducing position. These enzymes define major elements that control the structure of both glycogen and starch.

Methods

The kinetic parameters of the branching enzyme of Rhodothermus obamensis (RoBE) were established after in vitro incubation with different branched or unbranched ¦Á-glucans of controlled structure.

Results

A minimal chain length of ten glucosyl units was required for the donor substrate to be recognized by RoBE that essentially produces branches of DP 3-8. We show that RoBE preferentially creates new branches by intermolecular mechanism. Branched glucans define better substrates for the enzyme leading to the formation of hyper-branched particles of 30-70 nm in diameter (dextrins). Interestingly, RoBE catalyzes an additional ¦Á-4-glucanotransferase activity not described so far for a member of the GH13 family.

Conclusions

RoBE is able to transfer ¦Á(1 ¡ú 4)-linked-glucan in C4 position (instead of C6 position for the branching activity) of a glucan to create new ¦Á(1 ¡ú 4) linkages yielding to the elongation of linear chains subsequently used for further branching. This result is a novel case for the thin border that exists between enzymes of the GH13 family.

General significance

This work reveals the original catalytic properties of the thermostable branching enzyme of R. obamensis. It defines new approach to produce highly branched ¦Á-glucan particles in vitro.