Assessment of the microbody luminal pH in the filamentous fungus Penicillium chrysogenum
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- 作者：van der Lende ; Ted R. ; Breeuwer ; Pieter ; Abee ; Tjakko ; Konings ; Wil N. ; Driessen ; Arnold J.M.
- 关键词：Organelle ; Microbody ; Peroxisome ; Vacuole ; Penicillin ; Penicillium chrysogenum
- 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
- 出版年：2002
- 出版时间：April 3, 2002
- 年：2002
- 卷：1589
- 期：2
- 页码：104-111
- 全文大小：455 K
文摘
The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically active penicillins have to cross one or more membranes. The final enzymatic step that is mediated by acyltransferase takes place in a microbody. The pH of the microbody lumen in penicillin producing cells has been determined with fluorescent probes and mutants of the green fluorescent protein and found to be slightly alkaline.